Biochemistry · Nutritional Deficiency

Vitamin B12 Test

Measures Cobalamin levels in blood. B12 deficiency is especially common in vegetarians and causes fatigue, nerve damage, and megaloblastic anaemia.

Fasting: Not Required Report: Same Day Sample: Serum

Why Vitamin B12 Matters

Vitamin B12 (Cobalamin) is a water-soluble vitamin that the body cannot produce on its own — it must come entirely from diet or supplements. It is indispensable for myelin sheath formation (the protective coating around nerves), DNA synthesis, red blood cell maturation, and neurological health. Since B12 is found almost exclusively in animal-derived foods, vegetarians, vegans, and the elderly face the highest deficiency risk.

Critically, the body stores B12 in the liver for years — so deficiency often develops slowly and silently. By the time symptoms appear, nerve damage may already be irreversible. Early detection through blood testing is therefore essential.

Reference Ranges

Level (pg/mL)StatusClinical Significance
< 150 pg/mLSevere DeficiencyNerve damage, megaloblastic anaemia
150–200 pg/mLBorderline LowSymptomatic deficiency possible
200–900 pg/mLNormalAdequate B12 levels
> 900 pg/mLHigh (investigate cause)Liver disease, myeloproliferative disorders

Top Dietary Sources of Vitamin B12

Food SourceApprox. B12 ContentNote
Clams / Shellfish84 µg / 100gRichest natural source
Beef Liver70 µg / 100gExcellent source
Fish (Sardines, Salmon)8–12 µg / 100gGood non-meat source
Eggs1.1 µg / 100gModerate — absorbed well
Milk / Dairy0.4–0.9 µg / 100gOnly vegetarian source
Fortified FoodsVariesCereals, plant milks — check label

Who Is Most at Risk?

Vegetarians and vegans (no animal foods)
Adults over 50 (reduced absorption)
Patients on long-term Metformin (diabetes drug)
Patients on antacids / PPIs (omeprazole etc.)
Symptoms of numbness, tingling in hands/feet
Unexplained fatigue, memory problems

Preparation

No fasting required. If you take B12 supplements or B12 injections, inform your doctor — ideally wait a few days after an injection to get a meaningful result. The test can be done at any time of day.

Test Price at Velys Hospital

₹150

Inclusive of collection  ·  Same-day report

Book Vitamin B12 Test at Velys Hospital

No fasting needed · Walk-in available · Same-day results · Ahmedabad